Abstract Title

Detection of BHMT in Oligodendrocytes

Abstract

Multiple sclerosis (MS) is a neurodegenerative disease in which the body’s immune system attacks the nervous system leading to degradation of the myelin sheath surrounding nerve cells. Methionine metabolism is a pathway that is negatively affected in MS. In MS, inflammation and increased reactive oxygen species lead to a buildup of homocysteine which can cause complications. Further, SAM is down-regulated in MS causing decreased methylation and subsequent gene expression. To counter this problem, BHMT is an enzyme that is activated by betaine and helps prevent the accumulation of homocysteine and is therefore able to proceed with the conversion into methionine. BHMT is present in oligodendrocytes but the reason is not yet known. The focus of this project is to look at BHMT expression and potential co-localization with other proteins through immunocytochemistry. This information will give us a better understanding of BHMT and its role in oligodendrocytes.

Modified Abstract

Multiple sclerosis (MS) is a neurodegenerative disease in which the body’s immune system attacks the nervous system leading to degradation of the myelin sheath surrounding nerve cells. In MS, inflammation and increased reactive oxygen species lead to a buildup of homocysteine which can be prevented by activation of BHMT by betaine. BHMT is present in oligodendrocytes which may be neuroprotective. The focus of this project is to look at BHMT expression and potential co-localization with other proteins through immunocytochemistry.

Research Category

Biomedical Sciences

Author Information

Christian RichardsonFollow

Primary Author's Major

Biochemistry

Mentor #1 Information

Dr. Jennifer McDonough

McDonough

Mentor #2 Information

Sarah Sternbach

Sternbach

Mentor #3 Information

Dr. Earnest Freeman

Freeman

Presentation Format

Poster

Start Date

April 2019

Research Area

Medical Biochemistry | Medical Cell Biology | Nervous System Diseases

This document is currently not available here.

Share

COinS
 
Apr 9th, 1:00 PM

Detection of BHMT in Oligodendrocytes

Multiple sclerosis (MS) is a neurodegenerative disease in which the body’s immune system attacks the nervous system leading to degradation of the myelin sheath surrounding nerve cells. Methionine metabolism is a pathway that is negatively affected in MS. In MS, inflammation and increased reactive oxygen species lead to a buildup of homocysteine which can cause complications. Further, SAM is down-regulated in MS causing decreased methylation and subsequent gene expression. To counter this problem, BHMT is an enzyme that is activated by betaine and helps prevent the accumulation of homocysteine and is therefore able to proceed with the conversion into methionine. BHMT is present in oligodendrocytes but the reason is not yet known. The focus of this project is to look at BHMT expression and potential co-localization with other proteins through immunocytochemistry. This information will give us a better understanding of BHMT and its role in oligodendrocytes.