Abstract Title

Characterization of Luteinizing Hormone Location and Production in the Brain

Abstract

Luteinizing hormone (LH) is a heterodimeric glycoprotein gonadotropin that has long been known to play a critical role in reproduction and has recently been linked to changes in cognition and plasticity, especially as LH levels change in the aging process. While researchers have begun to understand some of the effects luteinizing hormone has in the brain, much remains to be explored. Specifically, studies have identified LH protein in several brain regions but it is not clear where it is produced. This study uses hybridization chain reaction in situ hybridization (HCR-FISH) in the mouse brain to identify and localize LH mRNA transcripts. In situ results were combined with immunohistochemistry and rtPCR results to provide a cohesive understanding of where LH is produced versus distributed. Furthermore, analysis of single cell RNA sequencing data identifies what types of cells are responsible for producing LH in regions of interest and suggests possible mechanisms for LH involvement in plasticity. This localization provides a foundation for further experiments to understand the mechanism by which LH affects cognition and plasticity.

Modified Abstract

Luteinizing hormone (LH) is a heterodimeric glycoprotein gonadotropin that plays a critical role in reproduction and has recently been linked to changes in cognition and plasticity, especially as LH levels change during aging. Studies have identified LH protein in several brain regions but it is not clear where it is produced versus where it has been transported from another region. This study uses molecular techniques in the mouse brain to identify and localize LH mRNA transcripts. Furthermore, analysis of RNA sequencing data identifies what types of cells are responsible for producing LH and suggests possible mechanisms for LH involvement in plasticity. This provides a foundation for further experiments to understand the mechanism by which LH affects cognition and plasticity.

Research Category

Biomedical Sciences

Primary Author's Major

Biology

Mentor #1 Information

Gemma

Casadesus-Smith

Presentation Format

Poster

Start Date

April 2019

Research Area

Bioinformatics | Molecular and Cellular Neuroscience

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Apr 9th, 1:00 PM

Characterization of Luteinizing Hormone Location and Production in the Brain

Luteinizing hormone (LH) is a heterodimeric glycoprotein gonadotropin that has long been known to play a critical role in reproduction and has recently been linked to changes in cognition and plasticity, especially as LH levels change in the aging process. While researchers have begun to understand some of the effects luteinizing hormone has in the brain, much remains to be explored. Specifically, studies have identified LH protein in several brain regions but it is not clear where it is produced. This study uses hybridization chain reaction in situ hybridization (HCR-FISH) in the mouse brain to identify and localize LH mRNA transcripts. In situ results were combined with immunohistochemistry and rtPCR results to provide a cohesive understanding of where LH is produced versus distributed. Furthermore, analysis of single cell RNA sequencing data identifies what types of cells are responsible for producing LH in regions of interest and suggests possible mechanisms for LH involvement in plasticity. This localization provides a foundation for further experiments to understand the mechanism by which LH affects cognition and plasticity.