Abstract Title

Role of Hemoglobin in the Nucleus of Neurons

Abstract

Expression of hemoglobin has been found to be expressed in neurons in both humans and rodents. In certain parts of the brain, specifically in the cortex, hippocampus, and substantia nigra, hemoglobin alpha and beta subunit mRNAs and proteins have been found. Both of the hemoglobin subunits, Hba and Hbb, are localized in the cytoplasm but only Hbb is found in the nucleus. Another study found that Hba immunoreactivity in mouse neurons from the cortical and hippocampal neurons in axons and dendrites but observed no Hba in the nucleus. We identified Hbb interacting proteins by performing co-immunoprecipitation and then liquid chromatography-mass spectrometry. The results of the co-IP and LC-MS showed there is a relationship between Hbb and both mitochondrial and nuclear proteins including histones in both rat and human brain primary neurons. Combining the results of our research with previous studies show promising results that could reveal how the expression of hemoglobin and the localization of hemoglobin in the brain changes with different environment conditions. These findings are important to understand the pathology of neurodegenerative diseases multiple sclerosis (MS), Alzheimer’s disease (AD), and Parkinson’s disease because each disease has been linked to the misexpression or subcellular localization of neuronal hemoglobin. To better understand the consequences of altered hemoglobin I am studying hemoglobin subcellular localization and the effects of inflammation.

Modified Abstract

Expression of hemoglobin has been found to be expressed in neurons in both humans and rodents. In certain parts of the brain hemoglobin alpha and beta subunit mRNAs and proteins have been found. Both of the hemoglobin subunits, Hba and Hbb, are localized in the cytoplasm but only Hbb is found in the nucleus. Our results showed showed there is a relationship between Hbb and histone methylation. I am investigation the mechanisms of Hbb translocation into and out of the nucleus.

Research Category

Biomedical Sciences

Author Information

Joe HinesFollow

Primary Author's Major

Biology

Mentor #1 Information

Dr. McDonough

Presentation Format

Poster

Start Date

5-4-2018 1:00 PM

Research Area

Cell Biology | Molecular and Cellular Neuroscience

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Apr 5th, 1:00 PM

Role of Hemoglobin in the Nucleus of Neurons

Expression of hemoglobin has been found to be expressed in neurons in both humans and rodents. In certain parts of the brain, specifically in the cortex, hippocampus, and substantia nigra, hemoglobin alpha and beta subunit mRNAs and proteins have been found. Both of the hemoglobin subunits, Hba and Hbb, are localized in the cytoplasm but only Hbb is found in the nucleus. Another study found that Hba immunoreactivity in mouse neurons from the cortical and hippocampal neurons in axons and dendrites but observed no Hba in the nucleus. We identified Hbb interacting proteins by performing co-immunoprecipitation and then liquid chromatography-mass spectrometry. The results of the co-IP and LC-MS showed there is a relationship between Hbb and both mitochondrial and nuclear proteins including histones in both rat and human brain primary neurons. Combining the results of our research with previous studies show promising results that could reveal how the expression of hemoglobin and the localization of hemoglobin in the brain changes with different environment conditions. These findings are important to understand the pathology of neurodegenerative diseases multiple sclerosis (MS), Alzheimer’s disease (AD), and Parkinson’s disease because each disease has been linked to the misexpression or subcellular localization of neuronal hemoglobin. To better understand the consequences of altered hemoglobin I am studying hemoglobin subcellular localization and the effects of inflammation.