Author(s) | |
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Abstract |
Acute Myeloid Leukemia (AML) is a cancer of the bone marrow (BM) and blood in which the BM produces abnormal myeloblasts. Vascular Endothelial Growth Factor A (VEGF-A) induces blood vessel formation that contributes to tumor growth, and inhibitors of VEGF-A are used clinically. However cancer cells acquire resistance to these drugs leading to recurrence, so we are exploring alternative pathways that could lead to novel anti-VEGF-A therapeutics. Recent studies using RNA-seq analysis have shown the importance of alternatively spliced isoforms in cancer, particularly those encoding proteins with different functions. With our interest in adult AML we have generated a computational pipeline to analyze RNA-seq databases to compare the isoform expression of VEGF-A in AML BM samples with that in healthy BM samples. We asked whether VEGF-A was highly expressed in AML and whether it’s alternative mRNA splice forms were differentially expressed in leukemia BM cells compared to normal BM cells. We have successfully identified altered VEGF-A isoform expression in adult AML but not pediatric AML. This suggests different cancer processes are prevalent in adult vs pediatric disease and warrants a further examination of other cancer types. |
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Publication Date |
2018-04-05
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Contributor(s) |
Faculty Mentor
Dr. Gail Fraizer Dr. Helen Piontkivska
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Subject | |
Modified Abstract |
Vascular Endothelial Growth Factor A (VEGF-A) induces the growth of blood vessels contributing to tumor growth in various cancers. Inhibitors of VEGF-A are used clinically, however cancer cells acquire resistance leading to recurrence, and so we are exploring alternative pathways that could lead to novel anti-VEGF-A therapeutics. With our interest in adult AML we have generated a computational pipeline to analyze RNA-seq data to compare VEGF-A isoform expression. We asked whether VEGF-A was highly expressed in AML and whether its isoforms were differentially expressed in AML vs. normal bone marrow data. We have successfully identified altered VEGF-A isoform expression in adult AML but not pediatric. This suggests different cancer processes are prevalent in adult vs pediatric disease and warrants further examination. |
Permalink | https://oaks.kent.edu/ugresearch/2018/2018all/131 |
VEGF-A isoform ratios in Acute Myeloid Leukemia
Johnson, E., & Plonski, N.-M. (2018). VEGF-A isoform ratios in Acute Myeloid Leukemia (1–). https://oaks.kent.edu/node/5591
Johnson, Emily, and Noel-Marie Plonski. 2018. “VEGF-A Isoform Ratios in Acute Myeloid Leukemia”. https://oaks.kent.edu/node/5591.
Johnson, Emily, and Noel-Marie Plonski. VEGF-A Isoform Ratios in Acute Myeloid Leukemia. 5 Apr. 2018, https://oaks.kent.edu/node/5591.