Abstract Title

Cell Proliferation, Viability and Cyto-toxicity Testing on Neuroblastoma Cells with the Use of Liquid Crystal Elastomers

Abstract

Amber Hydash1, Senay Ustunel1-3, Marianne E. Prévôt 2, Naveen Singhal1, Elda Hegmann1-3 and Robert J. Clements1*

1 Department of Biological Sciences, Kent State University, Kent, OH, USA

2 Liquid Crystal Institute, Kent State University, Kent, OH, USA

3 Chemical Physics Interdisciplinary Program, Kent State University, Kent, OH, USA

In vitro studies evaluating neuronal function using cell culture techniques are an invaluable tool. However two-dimensional (2D) studies do not provide accurate details regarding spatial neuronal interactions. We present a platform for long term study of neural networks in vitro using three-dimensional (3D) liquid crystal elastomer (LCE) foams as scaffolds which have proven to be non-cytotoxic to soft tissue cell lines such as, human neuroblastoma cells (SH-SY5Y) and support cell growth for over two months. Treating neurons with retinoic acid (RA) after four weeks of maturation results in an increased neurite length as observed under confocal microscopy. In order to stimulate myelination of neuronal elements in the 3D cultures – glial cells, which support and protect neurons, were seeded on neuroblastoma containing LCE’s. Here we present cell proliferation, viability and cytotoxicity tests assessed for cells grown on the LCE scaffolds. The development of the co-culture will lead to a better representation and understanding of spatial tissue interactions and a suitable method for evaluating therapies for neurodegenerative states.

Modified Abstract

In vitro studies evaluating neuronal function using cell culture techniques are an invaluable tool. However two-dimensional (2D) studies do not provide accurate details regarding spatial neuronal interactions. We present a platform for long term study of neural networks in vitro using three-dimensional (3D) liquid crystal elastomer (LCE) foams as scaffolds which have proven to be non-cytotoxic to soft tissue cell lines such as, human neuroblastoma cells (SH-SY5Y) and support cell growth for over two months. Treating neurons with retinoic acid (RA) after four weeks of maturation results in an increased neurite length as observed under confocal microscopy. In order to stimulate myelination of neuronal elements in the 3D cultures – glial cells, which support and protect neurons, were seeded on neuroblastoma containing LCE’s. Here we present cell proliferation, viability and cytotoxicity tests assessed for cells grown on the LCE scaffolds. The development of the co-culture will lead to a better representation and understanding of spatial tissue interactions and a suitable method for evaluating therapies for neurodegenerative states.

Research Category

Biomedical Sciences

Author Information

Amber HydashFollow

Primary Author's Major

Biology

Mentor #1 Information

Senay Ustunel

Mentor #2 Information

Dr. Robert Clements

Presentation Format

Poster

Start Date

5-4-2018 1:00 PM

Research Area

Cell Biology | Molecular Biology | Other Neuroscience and Neurobiology | Structural Biology

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Apr 5th, 1:00 PM

Cell Proliferation, Viability and Cyto-toxicity Testing on Neuroblastoma Cells with the Use of Liquid Crystal Elastomers

Amber Hydash1, Senay Ustunel1-3, Marianne E. Prévôt 2, Naveen Singhal1, Elda Hegmann1-3 and Robert J. Clements1*

1 Department of Biological Sciences, Kent State University, Kent, OH, USA

2 Liquid Crystal Institute, Kent State University, Kent, OH, USA

3 Chemical Physics Interdisciplinary Program, Kent State University, Kent, OH, USA

In vitro studies evaluating neuronal function using cell culture techniques are an invaluable tool. However two-dimensional (2D) studies do not provide accurate details regarding spatial neuronal interactions. We present a platform for long term study of neural networks in vitro using three-dimensional (3D) liquid crystal elastomer (LCE) foams as scaffolds which have proven to be non-cytotoxic to soft tissue cell lines such as, human neuroblastoma cells (SH-SY5Y) and support cell growth for over two months. Treating neurons with retinoic acid (RA) after four weeks of maturation results in an increased neurite length as observed under confocal microscopy. In order to stimulate myelination of neuronal elements in the 3D cultures – glial cells, which support and protect neurons, were seeded on neuroblastoma containing LCE’s. Here we present cell proliferation, viability and cytotoxicity tests assessed for cells grown on the LCE scaffolds. The development of the co-culture will lead to a better representation and understanding of spatial tissue interactions and a suitable method for evaluating therapies for neurodegenerative states.