Event Title

The Effects of Diet on Lipid Composition in Gypsy Moth Caterpillars (Lymantria dispar dispar) and their Subsequent Ability to Neutralize Toxic Oak Tannins

Location

101 Science & Nursing Building

Start Date

27-4-2018 10:00 AM

End Date

27-4-2018 10:30 AM

Description

Gypsy moth caterpillars (Lymantria dispar dispar) produce high concentrations of lysophosphatidylcholine, a lipoprotein necessary to neutralize toxic oak tree (Quercus spp.) tannins commonly found in their diet, hereby aiding their growth and survival on a common host. Nutrient ratios (protein to carbohydrates) of the caterpillar diet may affect lipoprotein composition in the hemolymph. In this study, we reared caterpillars on diets of varying nutrient ratios as well as varying concentrations of tannic acid. We hypothesized that diets containing higher protein: carbohydrate ratios would allow the caterpillars to produce higher concentrations of lipoproteins, such as lysophosphatidylcholine. Hemolymph was extracted from caterpillars, and thin layer chromatography followed by phosphate analysis were utilized to determine lipoprotein presence and abundance. This study provides insights on toxin neutralization of a generalist herbivore pest and offers possible new mechanisms for forest pest management strategies.

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Apr 27th, 10:00 AM Apr 27th, 10:30 AM

The Effects of Diet on Lipid Composition in Gypsy Moth Caterpillars (Lymantria dispar dispar) and their Subsequent Ability to Neutralize Toxic Oak Tannins

101 Science & Nursing Building

Gypsy moth caterpillars (Lymantria dispar dispar) produce high concentrations of lysophosphatidylcholine, a lipoprotein necessary to neutralize toxic oak tree (Quercus spp.) tannins commonly found in their diet, hereby aiding their growth and survival on a common host. Nutrient ratios (protein to carbohydrates) of the caterpillar diet may affect lipoprotein composition in the hemolymph. In this study, we reared caterpillars on diets of varying nutrient ratios as well as varying concentrations of tannic acid. We hypothesized that diets containing higher protein: carbohydrate ratios would allow the caterpillars to produce higher concentrations of lipoproteins, such as lysophosphatidylcholine. Hemolymph was extracted from caterpillars, and thin layer chromatography followed by phosphate analysis were utilized to determine lipoprotein presence and abundance. This study provides insights on toxin neutralization of a generalist herbivore pest and offers possible new mechanisms for forest pest management strategies.