Title

Measurement of Bacterial Volume by Transmission-through-dye Imaging

Publication Title

Journal of Microbiological Methods

Publication Date

12-2011

Document Type

Article

DOI

10.1016/j.mimet.2011.08.019

Keywords

acid blue 9, biovolume, DAPI, E.coli, transmission-through-dye microscopy

Disciplines

Biology

Abstract

Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.


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