Title

α-Synemin Localizes to the M-band of the Sarcomere Through Interaction with the M10 Region of Titin

Publication Title

FEBS Letters

Publication Date

12-20-2014

Document Type

Article

DOI

10.1016/J.FEBSLET.2014.11.001

Keywords

synemin, A-kinase anchoring protein, protein kinase A, intermediate filament, signal transduction, cytoskeleton, IF, intermediate filament, AKAP, A-kinase anchoring protein, PKA, protein kinase A, R, regulatory subunits of PKA, C, catalytic subunits of PKA, Ig, immunoglobulin, TK, titin kinase, ASI, α-synemin specific insert

Disciplines

Biochemistry | Biology | Molecular Biology

Abstract

α-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein–protein interaction that regulates the sub-cellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere.

Publisher

Elsevier Science

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