Abstract Title

In vitro model of polymicrobial biofilm consisting of both S. aureus and P. aeruginosa

Abstract

Chronic wound infections are typically polymicrobial, consisting of multispecies bacteria including both Gram negative and Gram positive strains. Although planktonic view of infection could explain most acute infections, it has not been adequate for understanding the pathogenesis of chronic infections. Pseudomonas aeruginosa and Staphylococcus aureus are the two most common causes of chronic wound infections and are frequently found together. Studies suggest that dual infection by P. aeruginosa and S. aureus are more virulent and/or result in worse outcomes than infection by single species bacteria alone. Although they are frequently found together in human infections, P. aeruginosa has shown to quickly kill S. aureus when the two are grown together in planktonic co-cultures in vitro, which makes it difficult to develop an appropriate model that closely reflects polymicrobial infection in human chronic wounds. The objective of this study is to establish an in vitro model for polymicrobial biofilms consisting of both S. aureus and P. aeruginosa. This was achieved by inoculating them at different ratios and determining the optimal condition that results in the growth of both bacteria.

Modified Abstract

Pseudomonas aeruginosa and Staphylococcus aureus are the two most common causes of chronic wound infections and are frequently found together. Studies suggests that P. aeruginosa and S. aureus infect simultaneously resulting in worse outcomes than infection by single species bacteria alone. P. aeruginosa has shown to quickly kill S. aureus when the two are grown together in planktonic co-cultures in vitro, making it difficult to develop an appropriate model closely reflecting polymicrobial infection in human chronic wound. The objective of this study is to establish an in vitro model for polymicrobial biofilms consisting of both S. aureus and P. aeruginosa. This was achieved by inoculating them at different ratios and determine the optimal condition that results in growth of both bacteria.

Research Category

Biomedical Sciences

Primary Author's Major

Pre-Medicine/Pre-Osteopathy

Mentor #1 Information

Dr. Min-Ho Kim

Presentation Format

Poster

Start Date

21-3-2017 1:00 PM

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Research Area

Medical Cell Biology

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Mar 21st, 1:00 PM

In vitro model of polymicrobial biofilm consisting of both S. aureus and P. aeruginosa

Chronic wound infections are typically polymicrobial, consisting of multispecies bacteria including both Gram negative and Gram positive strains. Although planktonic view of infection could explain most acute infections, it has not been adequate for understanding the pathogenesis of chronic infections. Pseudomonas aeruginosa and Staphylococcus aureus are the two most common causes of chronic wound infections and are frequently found together. Studies suggest that dual infection by P. aeruginosa and S. aureus are more virulent and/or result in worse outcomes than infection by single species bacteria alone. Although they are frequently found together in human infections, P. aeruginosa has shown to quickly kill S. aureus when the two are grown together in planktonic co-cultures in vitro, which makes it difficult to develop an appropriate model that closely reflects polymicrobial infection in human chronic wounds. The objective of this study is to establish an in vitro model for polymicrobial biofilms consisting of both S. aureus and P. aeruginosa. This was achieved by inoculating them at different ratios and determining the optimal condition that results in the growth of both bacteria.