Abstract

Investigation of the Dynamics of Helix 18 Pseudoknot in 16S rRNA

Destiny Kaznoch, Kumudie Jayalath, Courtney Wolfe

Advisor Sanjaya Abeysirigunwardena

Department of Chemistry Biochemistry, Kent State University, Kent OH 44240

Abstract

RNA modifications are known to influence the local structure and thermodynamics of RNA. These nucleotide modifications are present in various RNAs including functionally important regions ribosomal RNA. A pseudouridine and 7-methyl guanine modified nucleotides are present at positions 516 and 524 of 16S rRNA. My research is focused on understanding the structural and thermodynamic effects of these two modification observed in the helix 18 region of 16S rRNA. Specifically, we found that thermodynamics, pH and kinetics cause folding and unfolding of the helix 18 pseudoknot and these modified nucleotides influence its folding dynamics.

Modified Abstract

Investigation of the Dynamics of Helix 18 Pseudoknot in 16S rRNA

Destiny Kaznoch, Kumudie Jayalath, Courtney Wolfe and Sanjaya Abeysirigunwardena

Department of Chemistry Biochemistry, Kent State University, Kent OH 44240

Abstract

RNA modifications are known to influence the local structure and thermodynamics of RNA. These nucleotide modifications are present in various RNAs including functionally important regions ribosomal RNA. A pseudouridine and 7-methyl guanine modified nucleotides are present at positions 516 and 524 of 16S rRNA. My research is focused on understanding the structural and thermodynamic effects of these two modification observed in the helix 18 region of 16S rRNA. Specifically, I study the thermodynamics and kinetics of folding and unfolding of the helix 18 pseudoknot and how these modified nucleotides influence its folding dynamics.

Research Category

Physics/Chemisty/Liquid Crystal

Primary Author's Major

Biochemistry

Mentor #1 Information

Dr. Sanjaya Abeysirigunawardena

Presentation Format

Poster

Start Date

March 2016

Research Area

Biochemistry | Life Sciences

Included in

Biochemistry Commons

Share

COinS
 
Mar 15th, 1:00 PM

Investigation of the Dynamics of Helix 18 Pseudoknot in 16S rRNA

Investigation of the Dynamics of Helix 18 Pseudoknot in 16S rRNA

Destiny Kaznoch, Kumudie Jayalath, Courtney Wolfe

Advisor Sanjaya Abeysirigunwardena

Department of Chemistry Biochemistry, Kent State University, Kent OH 44240

Abstract

RNA modifications are known to influence the local structure and thermodynamics of RNA. These nucleotide modifications are present in various RNAs including functionally important regions ribosomal RNA. A pseudouridine and 7-methyl guanine modified nucleotides are present at positions 516 and 524 of 16S rRNA. My research is focused on understanding the structural and thermodynamic effects of these two modification observed in the helix 18 region of 16S rRNA. Specifically, we found that thermodynamics, pH and kinetics cause folding and unfolding of the helix 18 pseudoknot and these modified nucleotides influence its folding dynamics.