Abstract Title

Isolation of NAA in Mice Brain Tissue Simulating Multiple Sclerosis

Abstract

Multiple sclerosis (MS) is an inflammatory neurodegenerative disease. In MS the myelin, oligodendrocytes, axons, and neurons of the central nervous system (CNS) are destroyed resulting in neural conduction abnormality and disability. Dr. Freeman and Dr. McDonough’s research found that cells with defects in mitochondria coincided with decreased levels of the neuronal mitochondrial metabolite N-acetyl aspartate (NAA) in MS brain tissue. NAA is formed in neurons and sent to oligodendrocytes to synthesize acetate and create lipids that are used in myelin. They hypothesized that due to the defects in neuron mitochondria, this leads to a decrease in the composition of NAA, leading to changes in myelin production of MS. To test this hypothesis nuclear magnetic resonance (NMR) was used to identify and isolate NAA in MS simulated mice brain tissue.

Modified Abstract

Multiple sclerosis (MS) is an inflammatory neurodegenerative disease. In MS the myelin, oligodendrocytes, axons, and neurons of the central nervous system (CNS) are destroyed resulting in neural conduction abnormality and disability. Dr. Freeman and Dr. McDonough’s research found that cells with defects in mitochondria coincided with decreased levels of the neuronal mitochondrial metabolite N-acetyl aspartate (NAA) in MS brain tissue. NAA is formed in neurons and sent to oligodendrocytes to synthesize acetate and create lipids that are used in myelin. They hypothesized that due to the defects in neuron mitochondria, this leads to a decrease in the composition of NAA, leading to changes in myelin production of MS. To test this hypothesis mice subjects were used to simulate MS.

Research Category

Biology/Ecology

Primary Author's Major

Biochemistry

Mentor #1 Information

Dr. Jennifer A. McDonough

Mentor #2 Information

Dr. Naveen Singhal

Mentor #3 Information

Joe Thomas

Presentation Format

Poster

Start Date

March 2016

Research Area

Biochemistry

This document is currently not available here.

Share

COinS
 
Mar 15th, 1:00 PM

Isolation of NAA in Mice Brain Tissue Simulating Multiple Sclerosis

Multiple sclerosis (MS) is an inflammatory neurodegenerative disease. In MS the myelin, oligodendrocytes, axons, and neurons of the central nervous system (CNS) are destroyed resulting in neural conduction abnormality and disability. Dr. Freeman and Dr. McDonough’s research found that cells with defects in mitochondria coincided with decreased levels of the neuronal mitochondrial metabolite N-acetyl aspartate (NAA) in MS brain tissue. NAA is formed in neurons and sent to oligodendrocytes to synthesize acetate and create lipids that are used in myelin. They hypothesized that due to the defects in neuron mitochondria, this leads to a decrease in the composition of NAA, leading to changes in myelin production of MS. To test this hypothesis nuclear magnetic resonance (NMR) was used to identify and isolate NAA in MS simulated mice brain tissue.