Abstract Title

The Effect of Gelatin Microgel Stiffness on the Morphology of Encapsulated Mesenchymal Stem Cells

Abstract

The use of injectable hydrogel scaffolds for encapsulating stem cells has been studied as an effective approach for cell delivery for regenerative medicine. The objective of this study is to examine the effect of hydrogel stiffness on the morphology of encapsulated mesenchymal stem cells (MSCs). Mouse MSCs were encapsulated within gelatin microspheres using a water-in-oil phase bulk emulsification method and the extent of gel stiffness was controlled by changing crosslinking time (5, 30 and 60 minutes) of gelatin matrix. The change in MSC morphology and spreading within three-dimensional microgel space was quantified by measuring cell circularity and spreading. Results shows that MSCs encapsulated within low stiffness gels exhibited high circularity compared with those within high stiffness gels.

Research Category

Biomedical Sciences

Primary Author's Major

Biology

Mentor #1 Information

Dr. Min-Ho Kim

Mentor #2 Information

Dr. Baeckkyoung Sung

Presentation Format

Poster

Start Date

11-3-2015 1:00 PM

End Date

2015 5:00 PM

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Head shot

Group Biography- Shaffer, Sittek.docx (12 kB)
Group biography as Word document

Research Area

Biotechnology

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Mar 11th, 1:00 PM Jan 1st, 5:00 PM

The Effect of Gelatin Microgel Stiffness on the Morphology of Encapsulated Mesenchymal Stem Cells

The use of injectable hydrogel scaffolds for encapsulating stem cells has been studied as an effective approach for cell delivery for regenerative medicine. The objective of this study is to examine the effect of hydrogel stiffness on the morphology of encapsulated mesenchymal stem cells (MSCs). Mouse MSCs were encapsulated within gelatin microspheres using a water-in-oil phase bulk emulsification method and the extent of gel stiffness was controlled by changing crosslinking time (5, 30 and 60 minutes) of gelatin matrix. The change in MSC morphology and spreading within three-dimensional microgel space was quantified by measuring cell circularity and spreading. Results shows that MSCs encapsulated within low stiffness gels exhibited high circularity compared with those within high stiffness gels.