Location

101 Science & Nursing Building & Lobby

Start Date

28-4-2017 2:45 PM

End Date

28-4-2017 3:15 PM

Description

The genus Pseudomonas contains many species that range from harmless commensals to antibioticresistant pathogens. The most dangerous group is Pseudomonas aeruginosa, a leading cause of concern as the source of many antibiotic-resistant infections. Pseudomonas putida and Pseudomonas fluorescens were used in this study as they exhibit properties similar to those of P. aeruginosa without the issue of pathogenicity. Previous studies with non-pathogenic organisms such as Escherichia coli and Staphylococcus epidermis have shown an increase in antibiotic sensitivity when grown in the presence of sodium acetate, a known chemical stress agent, on complex media. This project is designed to study the effects of sodium acetate as the sole carbon source on antibiotic sensitivity of Pseudomonas putida and Pseudomonas fluorescens grown on M9 minimal medium. Antibiotic sensitivity is measured using the standard Kirby-Bauer method.

Comments

Rebekah Snyder is a senior at Kent State Tuscarawas, majoring in Veterinary Technology. After she graduates she plans to continue her education with a bachelor’s degree in biology and potentially graduate school. Her interests are in lab animal and large animal research. While not in school she enjoys volunteer work and spending time outdoors.

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Apr 28th, 2:45 PM Apr 28th, 3:15 PM

Investigation of Minimal Media and Chemical Stress on Antibiotic Sensitivity of Pseudomonas putida and Pseudomonas fluorescens

101 Science & Nursing Building & Lobby

The genus Pseudomonas contains many species that range from harmless commensals to antibioticresistant pathogens. The most dangerous group is Pseudomonas aeruginosa, a leading cause of concern as the source of many antibiotic-resistant infections. Pseudomonas putida and Pseudomonas fluorescens were used in this study as they exhibit properties similar to those of P. aeruginosa without the issue of pathogenicity. Previous studies with non-pathogenic organisms such as Escherichia coli and Staphylococcus epidermis have shown an increase in antibiotic sensitivity when grown in the presence of sodium acetate, a known chemical stress agent, on complex media. This project is designed to study the effects of sodium acetate as the sole carbon source on antibiotic sensitivity of Pseudomonas putida and Pseudomonas fluorescens grown on M9 minimal medium. Antibiotic sensitivity is measured using the standard Kirby-Bauer method.