Title

Three-Dimensional Imaging of Chemical Bond Orientation in Liquid Crystals by Coherent Anti-Stokes Raman Scattering Microscopy

Publication Title

Optics Express

Publication Date

10-17-2007

Document Type

Article

DOI

10.1364/OE.15.013585

Keywords

cars microscopy, multiplex cars, in-vivo, order, spectroscopy, generation, tissue, cells

Disciplines

Physics

Abstract

Liquid crystals are a class of industrially important materials whose optical properties make them useful particularly in display technology. Optical imaging of these materials provides information about their structure and physical properties. Coherent anti-Stokes Raman scattering (CARS) microscopy is used to provide three-dimensional chemical maps of liquid crystalline samples without the use of external labels. CARS is an optical imaging technique that derives contrast from Raman-active molecular vibrations in the sample. Compared to many other three-dimensional imaging techniques, CARS offers more rapid chemical characterization without the use of external dyes or contrast agents. The use of CARS to image chemical and orientational order in liquid crystals is demonstrated using several examples, and the limitations and benefits are discussed.

Comments

This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.15.013585 .Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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